Eric Wang

Perturbations of cerebral protein expression of rats with cirrhosis : validation of a proteomic study.

, Médecine, Université de Montréal

PREMIER

Direction:

Christopher Rose

2023 - 2023

Related Publications

Publications

Recurrent hepatic encephalopathy as a MELD exception point for liver transplantation.

Isaac Ruiz, Eric Wang, Mélanie Tremblay, YuShi. Wang, Geneviève Huard, Julien Bissonnette, Catherine Vincent, Chantal Bémeur, Christopher F. Rose.

Introduction: Hepatic encephalopathy (HE) is a complex neuropsychiatric syndrome arising from chronic liver disease (CLD). Itis characterized by cognitive impairments and associated with systemic and neurological inflammation. A promising strategy to mitigate cognitive deficits linked to HE is supplementation with omega-3 polyunsaturated fatty acids (3 PUFAs), known for their anti-inflammatory properties. The objective of this study is to evaluate the effect of fish oil rich in 3 PUFAs on memory and motor coordination in rats subjected to induced CLD. Methods: Rats underwent bile duct ligation (BDL) to induce CLD and HE or sham surgery (SHAM). Five days following surgery, rats received either fish oil supplementation (FO) or saline (SAL) by gavage daily for 4 weeks. Food intake and body weight was monitored weekly. Novel object recognition test (short/long-memory) and rotarod test (motor coordination) were assessed. Plasmatic cytokines were measured and liver injury biomarkers (aspartate and alanine aminotransferase (AST/ALT); bilirubin and albumin). Results: Regarding body weight, a global effect of supplementation was observed from week 3 to week 5 (p < 0.05) between SHAM groups and BDL groups. FO supplementation did not impact food intake in BDL rats as a significant decrease was noted in both BDL groups vs both SHAM groups at week 5 (p < 0.05). An improvement in short term memory was observed in BDL-FO vs BDL-SAL (p < 0.05). Regarding long-term memory, a global effect of supplementation was noted (p = 0.058) between SHAM groups and BDL groups. FO supplementation did not improve motor coordination with no difference between the BDL groups (p = 0.207). FO supplementation did not impact plasma pro-inflammatory cytokines (TNF-α/IL-1ꞵ) and CLD severity with no significant difference in liver injury biomarkers between BDL groups. Conclusion: 3 PUFA supplementation improves short-term memory in 5-week BDL rats. However, the benefit of FO is not associated with a reduction in systemic inflammation or liver disease severity. These findings highlight the therapeutic potential of 3 PUFAs in managing cognitive deficits associated with HE but the mechanism of action of 3 PUFAs in CLD remains unresolved.

$Impact du drainage ascitique sur l’incidence d’hospitalisations due à un épisode d’encéphalopathie hépatique chez les patients cirrhotiques avec ascite réfractaire.$

Impact du drainage ascitique sur l’incidence d’hospitalisations due à un épisode d’encéphalopathie hépatique chez les patients cirrhotiques avec ascite réfractaire.

James Gazetas, Isaac Ruiz, Mélanie Tremblay, Eric Wang, Chantal Bémeur, Christopher F. Rose.

Comparaison de la quantité de protéine aldolase C, cortactin et alpha-synucléine utilisant la méthode du western blot dans des hippocampes de rats avec une ligature du canal biliaire et des hippocampes de rats avec opération contrôle

Eric Wang, Farzaneh Tamnanloo, Chantal Bémeur, Christopher F. Rose.