Hepato-neuro lab

RT-PCR

Technique to detect and quantify mRNA.

Technique used to detect and quantify mRNA levels of specific genes in cells or tissues. mRNA is a messager RNA, used by cell to translated gene information encoded in DNA to proteins. Using an enzyme, transcriptase reverse, we first transcribe RNA into DNA. DNA is after amplified by an another enzyme, polymerase. Final product is separated by its size on a agarose gel and is visualized with a dye under ultra-violets rays.

Schematic representation of PCR

Schematic representation of PCR

Example of a typic agarose gel. In 1, a marker of known molecular weigth allow estimation of our sample

Example of a typic agarose gel. In 1, a marker of known molecular weigth allow estimation of our sample's fragment.

Data obtained by Mélanie Tremblay