Technique used to detect and quantify mRNA levels of specific genes in cells or tissues. mRNA is a messager RNA, used by cell to translated gene information encoded in DNA to proteins. Using an enzyme, transcriptase reverse, we first transcribe RNA into DNA. DNA is after amplified by an another enzyme, polymerase. Final product is separated by its size on a agarose gel and is visualized with a dye under ultra-violets rays.